RAM approachÀÇ ¸ñÀû
 
  • RAM approach¸¦ ÀÌ¿ëÇÑ À¯¿ë ±â´É¼º À¯ÀüÀÚÀÇ ´ë·® ¹ß±¼
  • À¯¿ë ±â´É¼º À¯ÀüÀÚÀÇ ÁöÀû ¼ÒÀ¯±Ç È®º¸/ licensing-out
  • À¯¿ë ±â´É¼º À¯ÀüÀÚÀÇ »ó¾÷Àû ÀÌ¿ë
    - Çʼö À¯ÀüÀÚ¸¦ disruptionÇÏ´Â chemicalÀ» ÇÕ¼ºÇÔÀ¸·Î½á »õ·Î¿î ³ó¾à Á¦Á¶
    - Transgenic seed »ý»ê
    - ÀÌ À¯ÀüÀÚ¸¦ ´Ù¸¥ °íºÎ°¡ ½Ä¹°¿¡ Àû¿ëÇÏ¿© È­ÈÑ µî¿¡ ÀÀ¿ë
 
RAM approachÀÇ Æ¯Â¡
 
  • Çʼö À¯ÀüÀÚÀÇ ±â´É¼º µ¿Á¤¿¡ ´Ù¸¥ ±â´É¼º À¯Àüü ¿¬±¸º¸´Ù ¸¹Àº ÀåÁ¡À» °¡Áø´Ù.
  • º¯ÀÌüÀÇ Ç¥ÇöÇüÀÌ ¿ì¼ºÀ¸·Î ¹ß»ý Ãʱ⿡ ½±°Ô º¯ÀÌü¸¦ µ¿Á¤ÇÒ ¼ö ÀÖ´Ù.
    cf. insertional mutagenesis´Â º¯ÀÌüÀÇ Ç¥ÇöÇüÀÌ ¿­¼ºÀ¸·Î ³ªÅ¸³².
  • Multigene family¿¡¼­µµ in vivo »óÅ·ΠƯÁ¤ À¯ÀüÀÚÀÇ ±â´ÉÀ» µ¿Á¤ÇÒ ¼ö ÀÖ´Ù.
  • Çʼö À¯ÀüÀÚÀÇ antisense disruptionÀº º¯ÀÌüÀÇ È¸º¹µÇÁö ¾Ê´Â Ä¡»ç¸¦ À̲ø °ÍÀÌ´Ù. ±×·¯¹Ç·Î inducible vectorÀÇ »ç¿ëÀÌ ÇʼöÀûÀÌ´Ù.
  • Simple PCR method¸¦ ÅëÇÏ¿© ½±°Ô À¯ÀüÀÚ¸¦ cloneÇÒ ¼ö ÀÖ´Ù

 
RAM approachÀÇ ¿ø¸®
  1. Construction of antisense cDNA library
2. Plant transformation
3. Selection of transgenic plants
4. Screening of mutants
5. Isolation of insert cDNA
6. Analysis of sequencing data for insert cDNA
7. Plant retransformation
 
 
 
 
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