Á¦³ë¸¶Àο¡¼­´Â ´Ù¾çÇÑ À¯ÀüÀÚ°ü·Ã ½ÇÇè ¼­ºñ½º¸¦ Á¦°øÇÕ´Ï´Ù. °í°´ÀÌ ¿øÇϽô À¯ÀüÀÚÀÇ ºÐ¸®, µ¿Á¤ ¹× ½Ä¹°ÇüÁúÀüȯüÀÇ À¯ÀüÀÚ º¯À̺ÎÀ§ÀÇ Å½»ö, À¯ÀüÀÚ ¹ßÇö ºÐ¼®±îÁö ´Ù³â°£ ÃàÀûµÈ ±â¼ú·ÂÀ» ¹ÙÅÁÀ¸·Î Á¤È®ÇÑ °á°ú¿Í °æÁ¦ÀûÀÎ °¡°ÝÀ¸·Î ½ÇÇè¼­ºñ½º¸¦ °ø±ÞÇØ µå¸³´Ï´Ù. »Ó¸¸ ¾Æ´Ï¶ó ±âŸ ½ÇÇè¿¡ °üÇؼ­µµ ÀÚ¼¼ÇÑ »ó´ã°ú ÀÇ·Ú°¡ °¡´ÉÇÕ´Ï´Ù.

¼­ºñ½ºÀÇ ¿¹·Î µ¿, ½Ä¹° À¯ÀüÀÚ °ü·Ã ±âº»ÀûÀÎ ÇмúÀû ½ÇÇè, À¯ÀüÀÚ º¯ÀÌü ºÐ¼®, ÀÓ»ó½Ã·á ºÐ¼®, ½Ã·áÁß À¯ÀüÀÚ º¯ÀÌü ÇÔ·® ºÐ¼®, À¯ÀüÀÚ ½Ã·á ºÐ¸®µîÀÌ ÀÖ½À´Ï´Ù.



(ÀϹÝÀûÀÎ PCR°ú T-vector cloning ±îÁö 1ÁÖÀÏ ¼Ò¿ä, ¿°±â¼­¿­ÀÇ È®ÀαîÁö´Â 2ÁÖ ¼Ò¿ä)

½Ã·áÁغñ
PCR
Cloning
Arabidopsis transfromation
Transient assay
Plasmid DNA
Genomic DNA
Total RNA
mRNA
amplification
RT-PCR
TAIL-PCR
Inverse-PCR
±âŸ
 
T-vector cloning
ÀÇ·ÚÀÚ°¡ ¿øÇÏ´ÂVector ¼±Á¤
Transformation
vector
Expressionvector

 

In planta transformation
T1 plant ¼±º°
T2 plant ¼±º°
T3 plant ¼±º°
Transformation È®ÀÎ
-PCR
- southern blot
-Northern blot
Expression vector cloning
Large prep
Protoplast transformation



   
Sample Á¢¼ö
»ç¿ëÇÏ´Â ½Ã·á¿¡ µû¶ó Á¢¼ö ¹æ¹ýÀÌ ´Ù¸¨´Ï´Ù.
¸ÕÀú ¹®ÀÇ ÇØÁÖ¼¼¿ä. (uniim@genomine.com)
 
´ã´çÀÚ¿Í »ó´ã
½ÇÇèÀÚÀÇ ½ÇÇè ¸ñÀû¿¡ µû¶ó cloning schemeÀÌ ´Þ¶óÁö¹Ç·Î ¸ÕÀú
»ó´ãÇØÁֽʽÿÀ. (uniim@genomine.com)
 
½Ã·á Áغñ
Á¶Á÷ 1gÀ» ±âÁØÀ¸·Î
Genomic DNA °æ¿ì 75,000¿ø
RNA °æ¿ì110,000¿ø
Plasmid 2,000¿ø
Primer Á¦ÀÛ
(ÀÇ·ÚÀÚÀÇ ¼±ÅûçÇ×ÀÔ´Ï´Ù. Á¦ÀÛ´ëÇ൵ °¡´ÉÇÕ´Ï´Ù.)
 
PCR
PCRÀÇ Á¾·ù°¡ ¸¹À¸³ª ÀϹÝÀûÀÎ primer 1set·Î ÁõÆøÇÏ´Â °æ¿ì À¯ÀüÀÚ
1°³´ç 70,000¿øÀÔ´Ï´Ù
 
T-Vector Other Vector

ÀÇ·ÚÀÚÀÇ ¸ñÀû¿¡ µû¶ó vector cloningÀÌ °áÁ¤µË´Ï´Ù.
ÀϹÝÀûÀ¸·Î PCRÇÏ¿© À¯ÀüÀÚ¸¦ È®º¸ÇÑ ÈÄ Ãß°¡ÀûÀÎ ´Ù¸¥ ½ÇÇè ÇÏ°íÀÚ
ÇÒ ¶§´Â T-vector cloning ÈÄ Sequence È®ÀÎÀ» ±ÇÀåÇÕ´Ï´Ù.
Vector cloning °æ¿ì 176,000¿øÀÔ´Ï´Ù.
½Ã·áÁغñºÎÅÍ vector 1 °³ÀÇ cloning ±îÁö ¿¬¼ÓÀûÀ¸·Î ÁøÇàÀÌ µÇ¸é
250,000¿ø/1set ÀÔ´Ï´Ù.
T-vector ÀÌ¿ÜÀÇ ´Ù¸¥ vector cloningÀÇ °æ¿ì ÀÇ·ÚÀÚ°¡ vector¸¦ Á¦°ø
ÇÏ¿©¾ß ÇÕ´Ï´Ù. ÀúÈñ¿¡°Ô ÀÇ·Ú ÇϽŠºÐÀÌ ´Ù¸¥ vector·Î ¿Å±â´Â °æ¿ì
°¡°Ý Á¶Á¤ÀÌ °¡´ÉÇÕ´Ï´Ù. uniim@genomine.com
Sequencing
(ÀÇ·ÚÀÚÀÇ ¼±ÅûçÇ×ÀÔ´Ï´Ù.)
 
Arabidopsis transformation Transient assay
 


 
 
 
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