2-DE Service Blotting
Image Analysis   Protein Identification
 
2-De Service
 

1st dimensional IEF

24cm length and non-linear IPG strip
Broad range(pH4-10), 2unit or 3unit ranges(pH4.5-6.5 / pH4-7 / pH6-8 / pH6-9

2nd dimensional SDS-PAGE 24cm x 20cm x 1mm
10-16% gradient gel

Staining MALDI compatible ammoniacal silver staining(detection limit 0.5~1ng)
Coomassie blue staining
Colloidal Coomassie staining
Sample requirement
  • Ammoniacal silver staining À» ±âÁØÀ¸·Î ÀÌ»óÀûÀÎ ´Ü¹éÁú ¹ßÇöºÐ¼®À» À§Çؼ­
    100-200ug/gel ÀÇ ´Ü¹éÁú ¾çÀ» ÇÊ¿ä·Î ÇÕ´Ï´Ù
  • Radio-labeled materials Àº º°µµ ¹®ÀÇ ¹Ù¶ø´Ï´Ù.
Sample shipping
  • Dry ice package »óÅ·Πexpress delivery
  • ¹Ýµå½Ã µµÂø ½ÃÁ¡±îÁö frozen »óÅ°¡ À¯ÁöµÇ¾î¾ß ÇÕ´Ï´Ù.
Blotting
 
electrotransfer of proteins to PVDF or nitrocellulose membranes
ºÐ¼®°á°ú´Â CD copy¿Í ¿ä±¸°¡ ÀÖÀ» °æ¿ì wet gel Á¦°øÇÕ´Ï´Ù.
½Å·Ú¼º ÀÖ´Â °á°úÇؼ®À» À§ÇÏ¿© 2DE ´Â 2gels/sample À» ±âº»À¸·Î ÇÏ°í ÀÖÀ¸¸ç,
»ùÇÃÀÇ Æ¯¼º¿¡ µû¶ó °í°´ÀÇ ¿äû¿¡ ÀÇÇØ Àú·ÅÇÑ ºñ¿ëÀ¸·Î 3-5gels/sample µµ °¡´ÉÇÕ´Ï´Ù.

 
 
 
Copyright 2003 Genomine,Inc. All rights reserved.